RESOURCES

Synthetic Physical Interaction data

These data come from many screens published over several years. Pubmed identification numbers are provided as column headers. Note that we include a few unpublished datasets - please contact us if you would like to publish these data, since they have not been through peer review.

Here is the Excel file. Each column is a different screen and each row is a different GFP strain.

All the data are expressed as Log Growth Ratios (LGRs). This metric is the natural log of the growth ratio. The growth ratio is the size of the control colonies divided by the size of the experiment colonies. Consequently, LGRs >0 indicate poor experiment growth relative to the control. LGRs<0 indicate better growth of the experiment relative to the control.

Here is a list of GFP strains that often come up in our screens and should probably be ignored - we call these “frequent flyers”.

Note that data published before 2020 may differ slightly from the original published data as the raw data scans were re-analysed as part of the development of our ScreenGarden software and expressed as LGRs (we originally used Z scores).

FociQuant - An imageJ/FIJI package for quantifying fluorescence foci in images.

FociQuant scripts run on the software package FIJI, a microscope image compatible version of ImageJ, download FIJI from this site.

Click here for the different FociQuant scripts, and here is the publication.